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Anti-phospho-EPH A3+A4+A5 ( Tyr779) antibody (bs-7555R)

Anti-phospho-EPH A3+A4+A5 ( Tyr779) antibody (bs-7555R)

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Anti-phospho-EPH A3+A4+A5 ( Tyr779) antibody

英文名称phospho-EPH A3+A4+A5 ( Tyr779)
中文名称磷酸化内皮细胞受体蛋白酪氨酸激酶A3+A4+A5抗体
别    名EPH A3+A4+A5 (phospho Y779 + Y779 + Y833); EPH A3+A4+A5 (phospho Y779); p-EPH A3+A4+A5 (phospho Y779); CEK7; EHK 1; EHK1; EK4; EK7; EK8; EPH homology kinase 1; EPH like kinase 4; EPH like kinase 7; EPH like kinase 8; Eph like tyrosine kinase 1; EPH receptor A3; EPH receptor A4; EPH receptor A5; Ephrin type A receptor 3; Ephrin type A receptor 4; Ephrin type A receptor 5; ETK; ETK1; HEK; HEK4; HEK7; HEK8; Human embryo kinase 1; Receptor protein tyrosine kinase HEK7; Receptor protein tyrosine kinase HEK8; SEK; TYRO1; TYRO1 protein tyrosine kinase; TYRO4; TYRO4 protein tyrosine kinase; Tyrosine protein kinase receptor EHK 1; Tyrosine protein kinase receptor ETK1; Tyrosine protein kinase receptor SEK; Tyrosine protein kinase TYRO1; EPHA3_HUMAN; EPHA4_HUMAN; EPHA5_HUMAN.  

DATASHEET

Host:Rabbit

Target Protein:phospho-EPH A3+A4+A5 ( Tyr779)

IR:Immunogen Range:AA(p-Y)TT

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2042

Swiss Prot:P29320

Source:KLH conjugated synthesised phosphopeptide derived from human around the phosphorylation site of EPH 3 Tyr779:AA(p-Y)TT 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:EphA3 is a member of the Ephrin (Eph) family. The Ephrins and Eph-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the Ephrin A (EphA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the Ephrin B (EphB) class, which are transmembrane proteins. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-EPH A3+A4+A5 (Tyr779)) Polyclonal Antibody, Unconjugated (bs-7555R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-EPH A3+A4+A5 (Tyr779)) Polyclonal Antibody, Unconjugated (bs-7555R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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