Anti-CHI3L2 antibody (bs-12358R)

Anti-CHI3L2 antibody

DATASHEET

Host:Rabbit

Target Protein:CHI3L2

IR:Immunogen Range:121-220/390

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1117

Swiss Prot:Q15782

Source:KLH conjugated synthetic peptide derived from human YKL39/CHI3L2:121-220/390 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:YKL-39 is a 390 amino acid cartilage protein that belongs to the chitinase family of chitin-fragmenting hydrolases. Highly expressed in chondrocytes (cartilage cells) and synoviocytes (fibroblastic cells that line joint cavities), YKL-39 binds glycan structures with high affinity. Although related to bacterial chitinases, YKL-39 lacks the characteristic glutamate active site and, thus, does not have enzymatic chitinase activity. Patients affected with rheumatoid arthritis (RA) have autoimmunity against YKL-39, suggesting that YKL-39 is involved in osteoarthritic and/or rheumatoid joint disease. Additionally, YKL-39 is upregulated in early degenerative cartilage diseases (such as RA) and may be a marker of chondrocyte activation in these autoimmune conditions.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CHI3L2) Polyclonal Antibody, Unconjugated (bs-12358R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.