Anti-phospho-SP1 (Thr739) antibody
| 英文名称 | phospho-SP1 (Thr739) |
| 中文名称 | 磷酸化转录生长因子SP1抗体 |
| 别 名 | SP1 (phospho T739); SP1 (phospho-Thr739); SP1 (phospho-T739); p-SP1 (phospho T453); p-TSFP1 (phospho T453); Sp1 transcription factor isoform a; TSFP1; TSFP 1; Specificity protein 1; Transcription factor Sp1; SP 1; SP1; Sp1 transcription factor; SP1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-SP1 (Thr739)
IR:Immunogen Range:TA(p-T)PS
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6667
Swiss Prot:P08047
Source:KLH conjugated synthesised phosphopeptide derived from human SP1 around the phosphorylation site of Thr739:TA(p-T)PS
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Cow
Horse
African Green Monkey.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-SP1 (Thr739) (bs-17137R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 75/90 kD
Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-SP1 (Thr739)) Polyclonal Antibody, Unconjugated (bs-17137R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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