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Anti-phospho-PDCD4 (Ser67) antibody (bs-12594R)

Anti-phospho-PDCD4 (Ser67) antibody (bs-12594R)

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Anti-phospho-PDCD4 (Ser67) antibody

英文名称phospho-PDCD4 (Ser67)
中文名称磷酸化凋亡相关蛋白4抗体
别    名PDCD4 (phospho S67); p-PDCD4 (phospho S67); Death up-regulated gene protein; Dug; H731; Ma3; MGC33046; MGC33047; Neoplastic transformation inhibitor; Neoplastic transformation inhibitor protein; Nuclear antigen H731; Nuclear antigen H731 like; Nuclear antigen H731 like protein; Nuclear antigen H731-like; PDCD 4; Pdcd4; PDCD4_HUMAN; Programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a; Protein MA-3; Tis; Topoisomerase-inhibitor suppressed protein. 

DATASHEET

Host:Rabbit

Target Protein:phospho-PDCD4 (Ser67)

IR:Immunogen Range:KN(p-S)SR

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:27250

Swiss Prot:Q53EL6

Source:KLH conjugated synthesised phosphopeptide derived from human PDCD4 around the phosphorylation site of Ser67:KN(p-S)SR 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-PDCD4 (Ser67)) Polyclonal Antibody, Unconjugated (bs-12594R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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