Anti-EB2 antibody
| 英文名称 | EB2 |
| 中文名称 | 微管相关末端结合蛋白2抗体 |
| 别 名 | APC binding protein EB2; APC-binding protein EB2; EB1; EB2; End binding protein 2; End-binding protein 2; H.sapiens mRNA for novel T cell activation protein; MAPRE2; MARE2_HUMAN; Microtubule associated protein RP/EB family member 2; Microtubule-associated protein RP/EB family member 2; T cell activation protein EB1 family. |
DATASHEET
Host:Rabbit
Target Protein:EB2
IR:Immunogen Range:101-200/327
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:10982
Swiss Prot:Q15555
Source:KLH conjugated synthetic peptide derived from human End binding protein 2:101-200/327
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. This protein is a microtubule-associated protein that is necessary for spindle symmetry during mitosis. It is thought to play a role in the tumorigenesis of colorectal cancers and the proliferative control of normal cells. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- EB2 (bs-7837R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EB2) Polyclonal Antibody, Unconjugated (bs-7837R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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