Anti-COX4 antibody (bs-10257R)

Anti-COX4 antibody 

DATASHEET

Host:Rabbit

Target Protein:COX4

IR:Immunogen Range:51-169/169

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1327

Swiss Prot:P13073

Source:KLH conjugated synthetic peptide derived from human COX4:51-169/169 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oJNCgen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: kidney (Mouse) Lysate at 40 ug
Primary: Anti-COX4 (bs-10257R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD

Sample:
heart (Mouse) Lysate at 40 ug
Primary: Anti-COX4 (Bs-10257R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD

Sample: kidney (Mouse) Lysate at 40 ug
Primary: Anti-COX4 (bs-10257R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Polyclonal Antibody, Unconjugated (bs-10257R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-COX4 Polyclonal Antibody, Unconjugated(bs-10257R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining