Anti-phospho-DNA PKcs (Ser2056) antibody
| 英文名称 | phospho-DNA PKcs (Ser2056) |
| 中文名称 | 磷酸化DNA依赖性蛋白激酶抗体 |
| 别 名 | DNA PK (phospho S2056); p-DNA PK (phospho S2056); PRKDC (phospho T2056); DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNA PKcs; DNPK1; p460; PRKDC; HYRC; HYRC1; Variant; DNAPK; DNAPK catalytic subunit; p350; PRKDC; Protein Kinase DNA Activated Catalytic Polypeptide; XRCC 7; XRCC7; PRKDC_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-DNA PKcs (Ser2056)
IR:Immunogen Range:YS(p-S)QD
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5591
Swiss Prot:P78527
Source:KLH conjugated synthesised phosphopeptide derived from human DNA PK around the phosphorylation site of Ser3191:YS(p-S)QD
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES

Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-DNA PK/PRKDC(Ser2056) Polyclonal Antibody, Unconjugated(bs-3734R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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