Anti-Phospho-MYPT1 (Thr696) antibody
| 英文名称 | Phospho-MYPT1 (Thr696) |
| 中文名称 | 磷酸化肌球蛋白磷酸酶抗体 |
| 别 名 | MYPT1 (Phospho Thr696); MYPT1 (Phospho T696); Myosin Phosphatase (phospho T696); Myosin Phosphatase (phospho Thr696); p-MYPT1(Thr696); Myosin Phosphatase; M130; MBS; MGC133042; Myosin phosphatase target subunit 1; Myosin phosphatase targeting subunit 1; MYPT 1; MYPT1; PPP1R12A; Protein phosphatase 1 regulatory inhibitor subunit 12A; Protein phosphatase 1 regulatory subunit 12A; Protein phosphatase myosin binding subunit; MYPT1_HUMAN; Myosin phosphatase-targeting subunit 1; Protein phosphatase myosin-binding subunit. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-MYPT1 (Thr696)
IR:Immunogen Range:RS(p-T)QG
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4659
Swiss Prot:O14974
Source:KLH conjugated synthesised phosphopeptide derived from human around the phosphorylation site of Ser696:RS(p-T)QG
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in non muscle cells. The guanosine triphosphate (GTP) bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho associated kinase (Rho kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Therefore Rho appears to inhibit myosin phosphatase through the action of Rho kinase.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
Flow-Cyt(1μg /Test)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: BRL-3A (Rat)cell Lysate at 40 ug
Primary: Anti-p-MYPT1(Thr696) (bs-3287R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 113kD
Observed band size: 120 kD
Sample: 293T (human)cell Lysate at 40 ug
Primary: Anti-p-MYPT1(Thr696) (bs-3287R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 113kD
Observed band size: 120 kD
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