Anti-VPS35 antibody (bs-12772R)

Anti-VPS35 antibody

DATASHEET

Host:Rabbit

Target Protein:VPS35

IR:Immunogen Range:621-720/796

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:55737

Swiss Prot:Q96QK1

Source:KLH conjugated synthetic peptide derived from human VPS35:621-720/796 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-VPS35 (bs-12772R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kD
Observed band size: 91 kD

Sample:
MOLT-4(Human) Cell Lysate at 30 ug
Primary: Anti-VPS35 (bs-12772R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kD
Observed band size: 91 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VPS35) Polyclonal Antibody, Unconjugated (bs-12772R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.