Anti-CTRP6 antibody (bs-14104R)

Anti-CTRP6 antibody

DATASHEET

Host:Rabbit

Target Protein:CTRP6

IR:Immunogen Range:151-259/259

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:114904

Swiss Prot:Q9BXI9

Source:KLH conjugated synthetic peptide derived from human CTRP6:151-259/259 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:CTRP6 is a 259 amino acid secreted glycoprotein consisting of an N-terminal signal peptide sequence followed by a collagen-like domain and a C-terminal globular domain. Predominantly expressed in eye and placenta, CTRP6 is also upregulated in obese mice. Along with CTRP1, CTRP2, CTRP3 and CTRP5, CTRP6 circulates in the blood and is thought to function as an endocrine hormone. There are three isoforms of CTRP6 that are produced as a result of alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
embryo (Mouse) Lysate at 40 ug
Primary: Anti-CTRP6 (bs-14104R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTRP6) Polyclonal Antibody, Unconjugated (bs-14104R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.