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Anti-Paxillin antibody (bs-3539R)

Anti-Paxillin antibody (bs-3539R)

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Anti-Paxillin antibody

英文名称Paxillin
中文名称桩蛋白Paxillin抗体
别    名Paired box protein Pax 1; PAX 1; PAX1; Paxillin alpha; PXN; PXN protein; FLJ16691; FLJ23042; FLJ16691; PAXI_HUMAN; Paxillin; PXN protein.  

DATASHEET

Host:Rabbit

Target Protein:Paxillin

IR:Immunogen Range:501-591/591

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5829

Swiss Prot:P49023

Source:KLH conjugated synthetic peptide derived from human Paxillin:501-591/591 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(3ug/Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
MCF-7(Human) Lysate at 40 ug
NIH/3T3(Mouse)Lysate at 40 ug
Primary: Anti-Paxillin (bs-3539R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 63 kD

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Paxillin) Polyclonal Antibody, Unconjugated (bs-3539R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: HT1080 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37°C for 20 min; Incubation: Anti-Paxillin Polyclonal Antibody, conjugated (bs-3539R-FITC) 1:50, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

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