Anti-DNAJC21 antibody (bs-14387R)

Anti-DNAJC21 antibody

DATASHEET

Host:Rabbit

Target Protein:DNAJC21

IR:Immunogen Range:11-120/531

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:134218

Swiss Prot:Q5F1R6

Source:KLH conjugated synthetic peptide derived from human DNAJC21:11-120/531 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:With the presence of the J domain defining a protein as a member, the DnaJ family has evolved with diverse cellular localization and functions and is one of the largest chaperone families. DnaJ heat-shock-induced proteins are derived from the bacterium Escherichia coli and are controlled by the htpR regulatory protein. DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis. Members of this family contain cysteine-rich regions composed of zinc fingers that form a peptide-binding domain responsible for chaperone function. DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. DNAJC21 (DnaJ homolog subfamily C member 21), also known as DNAJA5 or JJJ1, is a 531 amino acid protein that contains two C2H2-type zinc fingers and one J domain. Expressed in placenta, pancreas, kidney and brain, DNAJC21 may be a co-chaperone for HSP 70.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Zebrafish
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
293T (human)Cell Lysate at 40 ug
Cerebrum (mouse) Lysate at 40 ug
kidney (mouse) Lysate at 40 ug
pancreas (mouse) Lysate at 40 ug
Primary: Anti- DNAJC21(bs-14387R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62kD
Observed band size: 62kD

Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNAJC21) Polyclonal Antibody, Unconjugated (bs-14387R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.