Anti-HBcAg antibody (bs-15455R)

Anti-HBcAg antibody

DATASHEET

Host:Rabbit

Target Protein:HBcAg

IR:Immunogen Range:1-100/185

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:N/A

Swiss Prot:P03148

Source:KLH conjugated synthetic peptide derived from Hepatitis B Virus Core Antigen:1-100/185 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection.
The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
ICC(1:100)

Cross Reactive Species:Human
Hepatitis B Virus.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

HepG2-NTCP HBV infected; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (HBcAg) Polyclonal Antibody, Unconjugated (bs-15455R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (AF488,1:200) at 37°C for 90 minutes, DAPI (blue)was used to stain the cell nuclei.
Maria Francesca Cortese,PhD researcher in Vall d'Hebron Research Institute (VHIR) of Barcelona (Spain).