Anti-Spindlin 1 antibody (bs-0724R)

Anti-Spindlin 1 antibody 

DATASHEET

Host:Rabbit

Target Protein:Spindlin 1

IR:Immunogen Range:3-100/237

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10927

Swiss Prot:Q9Y657

Source:KLH conjugated synthetic peptide derived from human Spindin 1:3-100/237 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Spindlin 1 (Spindlin-like protein 2; SPIN-2 ) protein May play a role in cell-cycle regulation during the transition from gamete to embryo. Subcellular location in the Nucleus. Belongs to the SPIN/STSY family.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: Human Cervical Cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Spindlin 1 Polyclonal Antibody, Unconjugated(bs-0724R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining