Anti-phospho-alpha Adducin (Ser436) antibody (bs-15968R)

Anti-phospho-alpha Adducin (Ser436) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-alpha Adducin (Ser436)

IR:Immunogen Range:RH(p-S)FQ

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:118

Swiss Prot:P35611

Source:KLH conjugated synthesised phosphopeptide derived from human alpha Adducin around the phosphorylation site of Ser436:RH(p-S)FQ 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Adducins are a family of cytoskeleton proteins encoded by three genes (Alpha, Beta, Gamma). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and Adducins are central in the cortical actin network organization. Adducin Alpha is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin Alpha and Adducin Gamma is ubiquitous in contrast to the restricted expression of Adducin Beta. Adducin Beta is expressed at high levels in brain and hematopoietic tissues, such as bone marrow, in humans, and in spleen in mice.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Testis (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Molt-4(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-alpha Adducin (Ser436) (bs-15968R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 120 kD