Anti-Human IgM/Alexa Fluor 488 antibody (bs-0345G-AF488)

Anti-Human IgM/Alexa Fluor 488 antibody

Quantity size:

100ul

Concentration:

2mg/ml   Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences.

Also known as:

Goat Anti-Human IgM (Alexa Fluor? 488); Immunoglobulin M;

Specificity:

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Goat Polyclonal IgG, affinity purified by Protein G.

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Reacts with: hu.

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Immunogen: Native Human IgM.

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Predicted Molecular Weight: 900kDa.

Storage:

Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).

Application:

Flow-Cyt=1:100-1000  IF=1:100-1000  
Excitation spectrum: 495nm
Emission spectrum: 519nm
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.