Tags Positive Control Whole Cell Lysate (bs-41230P)

Tags Positive Control Whole Cell Lysate

Quantity size:

250ug

MW:

41kDa

Source:

Recombinant protein

Form:

lyophilized powde

Purity:

≥95% (Purified by HPLC)

Storage:

Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).

Application:

Immunology research

Background:

This E.coli lysate contains a 41 kd recombinant protein expressing 11 commonly used Tags which are fused to the N-terminus of the protein in the order of:
T7 HSV
C-Myc
VSV-g
Glu-Glu
V5
E-tag
FLAG (DDDDK)
S-tag
HA
6x His
GST

Tag sequences:
T7 = CMASMTGGQQMG
HSV = QPELAPEDPEDC
CMYC = EQKLISEEDLC
VSV-g = YTDIEMNRLGK
Glu-Glu = EYMPME
V5 = GKPIPNPLLGLDST
E-tag = GAPVPYPDPLEPR
FLAG = DYKDDDDK
HA = YPYDVPDYAC
6xHIS = HHHHHH

Lyophilised: After the lyophilised pellet is dissolved in 250 ul SDS-gel sample buffer (1 mg/ml protein lysate) it can be used direct for sample loading and then stored at –20°C. The sample can be reused several times.

Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags.

Also known as:

Tags Positive Control (T7; HSV; C-Myc; VSV-g; Glu-Glu; V5; E-tag; FLAG (DDDDK); S-tag; HA; His; GST); E. coli Tags Positive Control Whole Cell Lysate; E. coli Positive Control (Escherichia coli) Whole Cell Lysate; E. coli Tags Positive Control (Escherichia coli) Whole Cell Lysate; E.coli Tags Positive Control Whole Cell Lysate; Escherichiacoli Tags Positive Control Whole Cell Lysate; epitope tags;

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.