Anti-AKT1 antibody (bs-0115R)

Anti-AKT1 antibody

DATASHEET

Host:Rabbit

Target Protein:AKT1

IR:Immunogen Range:401-479/479

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:207

Swiss Prot:P31749

Source:KLH conjugated synthetic peptide derived from human PKB:401-479/479 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2μg/Test)
ICC(1:100)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-AKT1  (bs-0115R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti- AKT1 (bs- 0115R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/70 kD

Sample:
NIH/3T3 Cell (Mouse) Lysate at 40 ug
DU145 Cell (Human) Lysate at 40 ug
Primary: Anti-AKT1 (bs-0115R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 60 kD

Sample:
Placenta (Mouse) Lysate at 30 ug
Lung (Mouse) Lysate at 30 ug
Primary: Rabbit Anti-AKT1 (bs-0115R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size: 56 kD;
Observed band size: 60 kD;

Sample: Mcf-7 Cell Lysate at 40 ug
Primary: Rabbit Anti-AKT1 (bs-0115R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size: 56 kD;
Observed band size: 60 kD;

Sample:
Heart(Rat) Cell Lysate at 40 ug
Primary: Anti-AKT1 (bs-0115R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 63 kD

Sample:
Cerebrum(Mouse) Cell Lysate at 40 ug
Primary: Anti-AKT1 (bs-0115R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 63 kD

Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-AKT1 (bs-0115R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 63 kD

Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti- AKT1 (bs- 0115R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/70 kD

Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- AKT1 (bs- 0115R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/70 kD

Sample:
HL-60 Cell (Human) Lysate at 30 ug
Primary: Anti- AKT1 (bs- 0115R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/70 kD

Tissue/cell: mouse transplant lymphoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PKB Polyclonal Antibody, Unconjugated(bs-0115R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (AKT1) polyclonal Antibody, Unconjugated (bs-0115R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.