Anti-PPAR gamma antibody (bs-0530R)

Anti-PPAR gamma antibody

DATASHEET

Host:Rabbit

Target Protein:PPAR gamma

IR:Immunogen Range:101-200/505

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5468

Swiss Prot:P37231

Source:KLH conjugated synthetic peptide derived from human PPAR Gamma:101-200/505 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The PPAR gamma antibody mainly is exist in the white fat organization, the fat for the PPAR gamma is born, blood sugar stability, the disease respond, the artery gruel kind hardens to rise the important function with the tumor occurrence of etc. all, but concerning the PPAR gamma to bone of function is a new research heat to order in recent years.A PPAR of many researches report gamma was go together with the body is after activate can the function promote many capable cells divided to increase to living but repress the ossification cell to divide to cause the bone measure the decrease or bone softs toward the fat cell in the marrow, the PPAR gamma promotes the ability and bones that the fat cell divide metabolize closely related, the performance is increasing along with the growth marrow fat content of the age, the ossification cell metabolism the outcome reduce, the different construction PPAR gamma 2 have the important function.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Heart (Mouse) Lysate at 30 ug
Primary: Anti- PPAR gamma (bs-0530R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 51 kD

Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: Adipocyte (Rat) Lysate at 40 ug
Lane 3: Large intestine (Mouse) Lysate at 40 ug
Lane 4: Lung (Mouse) Lysate at 40 ug
Primary: Anti-PPAR gamma (bs-0530R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD

Sample:
Liver (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti- PPAR gamma (bs-0530R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 51 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-PPAR gamma (bs-0530R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD

Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-PPAR gamma (bs-0530R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD

Sample: Stomach (Mouse) Lysate at 30 ug
Primary: Rabbit Anti- PPAR gamma (bs-0530R) at 1:300 dilution;
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size:57 kD Observed band size:55 kD

Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPAR gamma) Polyclonal Antibody, Unconjugated (bs-0530R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PPAR Gamma Polyclonal Antibody, Unconjugated(bs-0530R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PPAR gamma) polyclonal Antibody, Unconjugated (bs-0530R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Tissue/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37°C for 20 min; Incubation: Anti- PPAR gamma Polyclonal Antibody, conjugated (bs-0530R-cy5) 1:100, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.