Anti-ICAM1/CD54 antibody (bs-0608R)

Anti-ICAM1/CD54 antibody

DATASHEET

Host:Rabbit

Target Protein:ICAM1/CD54

IR:Immunogen Range:201-300/537

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3383

Swiss Prot:P13597

Source:KLH conjugated synthetic peptide derived from human CD54:201-300/537 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Hela(Human) Cell Lysate at 30 ug
Hela KO ICAM1 (Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1/CD54 (bs-0608R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/69 kD

Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/69 kD

Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: MCF-7 (Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1/CD54 (bs-0608R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110/58 kD
Observed band size: 110/58 kD

Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD54 (bs-0608R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/69 kD

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD54/ICAM-1 Polyclonal Antibody, Unconjugated(bs-0608R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining