Anti-Phospho-NFKB p65 (Ser468) antibody
| 英文名称 | Phospho-NFKB p65 (Ser468) |
| 中文名称 | 磷酸化细胞核因子NF-κB p65抗体 |
| 别 名 | NF-kB p65 (phospho S468); p-NF-kB p65 (phospho S468); RELA(phospho S468); NF kB P65; NF-kB p65; NFKBp65; NF-κBp65; p65 NF kappaB; p65 NFkB; NFKBp65; RELA; Transcription Factor p65; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)); V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; v rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A; p65NFKB; Avian reticuloendotheliosis viral (v rel) oncogene homolog A; MGC131774; NFKB 3; NFKB3; Nuclear Factor NF Kappa B p65 Subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-NFKB p65 (Ser468)
IR:Immunogen Range:LA(p-S)VD
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5970
Swiss Prot:Q04206
Source:KLH conjugated Synthesised phosphopeptide derived from human NFKBp65 around the phosphorylation site of Ser468:LA(p-S)VD
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:100-200)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg /test)
ICC(1:100)
IF(1:100-200)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane1:HL-60 (Human) Whole Cell Lysate at 30 ug
Lane2:Lung (Mouse) Tissue Lysate at 30 ug
Primary: Anti-phospho-NFKB p65(Ser468) (bs-3485R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-Phospho-NFKB p65 (Ser468) (bs-3485R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-NFKB p65(Ser468) (bs-3485R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-NFKB p65(Ser468) (bs-3485R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-NFKB p65 (Ser468)) Polyclonal Antibody, Unconjugated (bs-3485R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-NFKB p65(Ser468)) Polyclonal Antibody, Unconjugated (bs-3485R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-NFKB p65 (Ser468)) polyclonal Antibody, Unconjugated (bs-3485R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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