Anti-HSP70 antibody (bs-0244R)

Anti-HSP70 antibody

DATASHEET

Host:Rabbit

Target Protein:HSP70

IR:Immunogen Range:500-600/641

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3303

Swiss Prot:P0DMV8

Source:KLH conjugated synthetic peptide derived from human HSP70:500-600/641 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
Flow-Cyt(1ug/Test)
ICC(1:100)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-HSP70 (bs-0244R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD

Sample: Bladder (Mouse) Lysate at 40 ug
Primary: Anti-HSP70 (bs-0244R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD

Sample: Lung (Mouse) Lysate at 40 ug
Primary: Anti-HSP70 (bs-0244R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD

Sample:
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: Epididymis (Mouse) Lysate at 40 ug
Lane 3: Seminal vesicle (Mouse) Lysate at 40 ug
Lane 4: Liver (Mouse) Lysate at 40 ug
Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 6: Testis (Rat) Lysate at 40 ug
Lane 7: Spleen (Rat) Lysate at 40 ug
Lane 8: Hela (Human) Cell Lysate at 30 ug
Lane 9: Jurkat (Human) Cell Lysate at 30 ug
Lane 10: HepG2 (Human) Cell Lysate at 30 ug
Lane 11: A549 (Human) Cell Lysate at 30 ug
Lane 12: MCF-7 (Human) Cell Lysate at 30 ug
Primary:
Anti-HSP70 (bs-0244R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 68 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (bs-0244R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (bs-0244R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (bs-0244R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP70) polyclonal Antibody, Unconjugated (bs-0244R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.