Anti-phospho-p38 MAPK (Thr180 + Tyr182) antibody
| 英文名称 | phospho-p38 MAPK (Thr180 + Tyr182) |
| 中文名称 | 磷酸化丝裂原活化蛋白激酶p38抗体 |
| 别 名 | p38 (phospho T180 + Y182); p-p38 (phospho T180 + Y182); p38 (phospho T180/Y182); CSAID Binding Protein 1; CSAID binding protein; CSAID-binding protein; Csaids binding protein; CSBP 1; CSBP 2; CSBP; CSBP1; CSBP2; CSPB 1; CSPB1; Cytokine suppressive anti inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; EXIP; MAP kinase 14; MAP kinase MXI2; MAP kinase p38 alpha; MAPK 14; MAPK14; MAX interacting protein 2; MAX-interacting protein 2; Mitogen Activated Protein Kinase 14; Mitogen activated protein kinase p38 alpha; Mitogen-activated protein kinase 14; Mitogen-activated protein kinase p38 alpha; MK14_HUMAN; Mxi 2; Mxi2; p38 ALPHA; p38; p38 MAP kinase; p38 MAPK; p38 mitogen activated protein kinase; p38ALPHA; p38alpha Exip; PRKM14; PRKM15; RK; SAPK 2A; SAPK2A; Stress Activated Protein Kinase 2A. |
DATASHEET
Host:Rabbit
Target Protein:phospho-p38 MAPK (Thr180 + Tyr182)
IR:Immunogen Range:EM(p-T)G(p-Y)VA
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1432
Swiss Prot:Q16539
Source:KLH conjugated Synthesised phosphopeptide derived from human p38 MAPK around the phosphorylation site of Thr180/Tyr182:EM(p-T)G(p-Y)VA
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases(MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
Flow-Cyt(1μg /test)
ICC(1:100)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Mcf-7 Cell Lysate at 30 ug
Primary: Anti- phospho-p38 MAPK (Thr180 + Tyr182) (bs-2210R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-p38 MAPK (Thr180 + Tyr182) (bs-2210R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-phospho-p38 MAPK (Thr180 + Tyr182) (bs-2210R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-p38 MAPK (Thr180 + Tyr182)) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-p38 MAPK (Thr180 + Tyr182)) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-p38 MAPK (Thr180 + Tyr182)) polyclonal Antibody, Unconjugated (bs-2210R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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