Anti-Phospho-PPAR Gamma (ser273) antibody (bs-4888R)

Anti-Phospho-PPAR Gamma (ser273) antibody

DATASHEET

Host:Rabbit

Target Protein:Phospho-PPAR Gamma (ser273)

IR:Immunogen Range:DK(p-S)PF

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5468

Swiss Prot:P37231

Source:KLH conjugated synthesised phosphopeptide derived from human PPAR Gamma around the phosphorylation site of ser273:DK(p-S)PF 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-p-PPAR Gamma (ser273) (bs-4888R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 55kD

Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-PPAR Gamma (ser273) (bs-4888R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD

Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: Adipocyte (Mouse) Lysate at 40 ug
Lane 3: Lung (Rat) Lysate at 40 ug
Primary: Anti-Phospho-PPAR Gamma (ser273) (bs-4888R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58’51 kD
Observed band size: 51 kD

Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Anti-Phospho-PPAR Gamma (ser273) (bs- 4888R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-PPAR Gamma(ser273) Polyclonal Antibody, Unconjugated(bs-4888R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-PPAR Gamma(ser273) Polyclonal Antibody, Unconjugated(bs-4888R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-PPAR Gamma (ser273)) polyclonal Antibody, Unconjugated (bs-4888R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.