Anti-GFAP antibody (bs-10950R)

Anti-GFAP antibody 

DATASHEET

Host:Rabbit

Target Protein:GFAP

IR:Immunogen Range:341-432/432

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2670

Swiss Prot:P14136

Source:KLH conjugated synthetic peptide derived from human GFAP:341-432/432 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IP(1:20-100)
IHC-P(1:100-500)
Flow-Cyt(3μg/Test)
ICC(1:100)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
U251(human) Cell Lysate at 40 ug
U87MG(human) Cell Lysate at 40 ug
BV2(mouse) Cell Lysate at 40 ug
Primary: Anti-GFAP (bs-10950R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

GFAP was immunoprecipitated from human hela cells lysate with bs-10950R at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: human hela cells lysate 10 μg (Input).
Lane 2: bs-10950R IP in human hela cells lysate.
Lane 3: native rabbit IgG IP in human hela cells lysate (negative control).
Secondary
All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GFAP (bs-10950R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- GFAP (bs-10950R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

Sample:
Cerebellum (Rat) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- GFAP (bs-10950R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD

Sample:Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-GFAP (bs-10950R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

Sample:SP2/0(human) Cell Lysate at 40 ug
Primary: Anti-GFAP (bs-10950R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GFAP) polyclonal Antibody, Unconjugated (bs-10950R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.