Anti-HSP60 antibody
| 英文名称 | HSP60 |
| 中文名称 | 热休克蛋白-60/groEL抗体 |
| 别 名 | HSP 60; HSP-60; 60 kDa chaperonin;60 kDa heat shock protein mitochondrial; 60kDa; cb863; Chaperonin 60; Chaperonin;Chaperonin, 60-KD; CPN 60; CPN60; fa04a05; fb22d10;fi27b05; GROEL; GroEL Homolog; GroEL, E. coli, homolog of; Heat shock 60kD protein 1 (chaperonin); Heat shock 60kD protein 1 chaperonin; heat shock 60kDa protein 1 (chaperonin); Heat Shock Protein 60; Heat shock protein 65; HLD4;Hsp 60;HSP 65; HSP65; HSPD 1; HSPD1; HuCHA60; id:ibd2197; Mitochondrial matrix protein P1; P60 lymphocyte protein; sb:cb144; Short heat shock protein 60 Hsp60s1; 60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; CH60_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:HSP60
IR:Immunogen Range:501-573/573
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3329
Swiss Prot:P10809
Source:KLH conjugated synthetic peptide derived from human HSP60:501-573/573
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Kidney (Mouse) Lysate at 30 ug
Primary: Anti- HSP60 (bs-0191R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Sample: Heart (Mouse) Lysate at 30 ug
Primary: Anti-HSP60 (bs-0191R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Adrenal gland (Mouse) Lysate at 40 ug
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 4: Adrenal gland (Rat) Lysate at 40 ug
Lane 5: Jurkat (Human) Cell Lysate at 30 ug
Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 ug
Lane 7: HepG2 (Human) Cell Lysate at 30 ug
Lane 8: Hela (Human) Cell Lysate at 30 ug
Lane 9: K562 (Human) Cell Lysate at 30 ug
Primary: Anti-HSP60 (bs-0191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- HSP60 (bs-0191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-HSP60 (bs-0191R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP60) Polyclonal Antibody, Unconjugated (bs-0191R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated(bs-0191R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated(bs-0191R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainin
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