Anti-Histone H3.1 antibody
| 英文名称 | Histone H3.1 |
| 中文名称 | 组蛋白H3.1抗体 |
| 别 名 | H3 histone family, member A; H3 histone family, member B; H3 histone family, member C; H3 histone family, member D; H3 histone family, member E; H3 histone family, member F; H3 histone family, member G; H3 histone family, member H; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3.1; H3.f; H3/A; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31_HUMAN; H3F1K; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1 cluster H3A; HIST1 cluster H3B; HIST1 cluster H3C; HIST1 cluster H3D; HIST1 cluster H3E; HIST1 cluster H3F; HIST1 cluster H3G; HIST1 cluster H3I; HIST1 cluster H3J; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; histone 1, H3b; Histone 1, H3c; Histone 1, H3e; histone 1, H3f; histone 1, H3g; histone 1, H3h; histone 1, H3i; histone 1, H3j; histone cluster 1, H3a; histone cluster 1, H3b; Histone cluster 1, H3c; Histone cluster 1, H3e; histone cluster 1, H3g; histone cluster 1, H3h; histone cluster 1, H3i; histone cluster 1, H3j; Histone gene cluster 1 H3A; Histone gene cluster 1 H3B; Histone gene cluster 1 H3E; Histone gene cluster 1 H3F; Histone gene cluster 1 H3G; Histone gene cluster 1, H3; Histone gene cluster 1, H3C; Histone gene cluster 1, H3D; Histone gene cluster 1, H3I; Histone gene cluster 1, H3IH; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l |
DATASHEET
Host:Rabbit
Target Protein:Histone H3.1
IR:Immunogen Range:71-136/136
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:8350
Swiss Prot:P68431
Source:KLH conjugated synthetic peptide derived from human Histone H3.1:71-136/136
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Rabbit
Sheep
Chimpanzee
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:A549 Cell (Human) Lysate at 40 ug
Primary: Anti-Histone H3.1(bs-17422R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15kD
Observed band size: 15kD
Sample:TT Cell (Human) Lysate at 40 ug
Primary: Anti-Histone H3.1(bs-17422R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15kD
Observed band size: 15kD
Sample:Stomach (Mouse) Lysate at 40 ug
Primary: Anti-Histone H3.1(bs-17422R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15kD
Observed band size: 15kD
Sample:U251 Cell (Human) Lysate at 40 ug
Primary: Anti-Histone H3.1(bs-17422R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15kD
Observed band size: 15kD
Sample:A431 Cell(Human)Lysate at 40 ug
Primary: Anti-Histone H3.1(bs-17422R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15kD
Observed band size: 15kD
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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