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Anti-alpha smooth muscle Actin antibody (bs-0189R)

Anti-alpha smooth muscle Actin antibody (bs-0189R)

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Anti-alpha smooth muscle Actin antibody

英文名称alpha smooth muscle Actin
中文名称肌动蛋白α/α-SMA/α Actin抗体
别    名alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5

DATASHEET

Host:Rabbit

Target Protein:alpha smooth muscle Actin

IR:Immunogen Range:301-375/375

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:59

Swiss Prot:P62736

Source:KLH conjugated synthetic peptide derived from human Actin alpha:301-375/375 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1μg /test)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Rabbit
Sheep
Fish
Guinea Pig
Hamster
Cat
HMt,Op.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:Kidney (Mouse) Lysate at 40 ug
Primary: Anti- alpha-SMA (bs-0189R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Sample:Ovary (Mouse) Lysate at 40 ug
Primary: Anti-alpha-SMA (bs-0189R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Sample: A549(Human) Lysate at 40 ug
Primary: Anti- alpha-SMA (bs-0189R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Sample:
Brain (Rat) Lysate at 40 ug
Kidney (Rat) Lysate at 40 ug
Primary: Anti-alpha-SMA(bs-0189R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Sample:
Brain (Rat) Lysate at 40 ug
Kidney (Rat) Lysate at 40 ug
Primary: Anti- alpha-SMA (bs-0189R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

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