Anti-Dnmt3a antibody (bs-0497R)

Anti-Dnmt3a antibody

DATASHEET

Host:Rabbit

Target Protein:Dnmt3a

IR:Immunogen Range:26-100/912

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1788

Swiss Prot:Q9Y6K1

Source:KLH conjugated synthetic peptide derived from human Dnmt3a:26-100/912 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The tumor organization has the DNA methylation disorder, including and damps cancer gene high methylation DNA with the cell multiplication cycle close related cancer gene low methylation the methyl transferase (Dnmt) participation methylation the formation (is mainly Dnmt3a and Dnmt3b) and the maintenance (is mainly Dnmt1) (isoform CRA_b) (DNA methyltransferase MmuIIIA) (DNA MTase MmuIIIA)

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 3: Testis (Rat) Lysate at 40 ug
Lane 4: 293T (Human) Cell Lysate at 30 ug
Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 6: Molt-4 (Human) Cell Lysate at 30 ug
Lane 7: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-Dnmt3a (bs-0497R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 120 kD

Sample:
A549(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Lovo(Human) Cell Lysate at 30 ug
Primary: Anti-Dnmt3a (bs-0497R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 120 kD

Sample:
Embryo(Mouse) Lysate at 40 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-Dnmt3a (bs-0497R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 120 kD

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dnmt3a) Polyclonal Antibody, Unconjugated (bs-0497R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Dnmt3a Polyclonal Antibody, Unconjugated(bs-0497R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Dnmt3a Polyclonal Antibody, Unconjugated(bs-0497R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining