Anti-GLUT4 antibody (bs-0384R)

Anti-GLUT4 antibody

DATASHEET

Host:Rabbit

Target Protein:GLUT4

IR:Immunogen Range:401-509/509

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6517

Swiss Prot:P14672

Source:KLH conjugated synthetic peptide derived from human GLUT4:401-509/509 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:GLUT4 is the facilitated glucose transporter expressed exclusively in adipocytes and muscle cells, and is also known as the "insulin-responsive" glucose transporter. GLUT4 translocates from an ill-defined intracellular compartment to the plasma membrane in response to insulin. The total cellular content of GLUT4 is significantly decreased in adipose cells from many patients with Type II diabetes mellitus, and animals with some types of experimental diabetes.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Heart(Rat) Cell Lysate at 40 ug
Primary: Anti-GLUT4 (bs-0384R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD

Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti- GLUT4 (bs-0384R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD

Sample:
Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Heart (Rat) Lysate at 40 ug
Lane 4: Muscle (Mouse) Lysate at 40 ug
Lane 5: Muscle (Rat) Lysate at 40 ug
Primary: Anti-GLUT4 (bs-0384R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(bs-0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(bs-0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (bs-0384R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.