Anti-Gamma-Adaptin antibody
| 英文名称 | Gamma-Adaptin |
| 中文名称 | 衔接蛋白γ/γ-Adaptin抗体 |
| 别 名 | Adapter-related protein complex 1 subunit gamma-1; Adaptor protein complex AP 1 gamma 1 subunit; Adaptor protein complex AP 1 subunit gamma 1; Adaptor protein complex AP-1 subunit gamma-1; Adaptor related protein complex 1 gamma 1 subunit; adaptor-related protein complex 1 gamma 1 subunit; ADTG; ADTG; AP 1 complex subunit gamma 1; AP-1 complex subunit gamma-1; AP1G1; AP1G1_HUMAN; CLAPG1; CLAPG1; Clathrin assembly protein complex 1 gamma 1 large chain; Clathrin assembly protein complex 1 gamma large chain; Clathrin assembly protein complex 1 gamma-1 large chain; Clathrin associated/assembly/adaptor protein large gamma 1; clathrin-associated/assembly/adaptor protein large gamma 1; Gamma1 adaptin; Gamma1-adaptin; Golgi adaptor HA1/AP1 adaptin gamma subunit; Golgi adaptor HA1/AP1 adaptin subunit gamma 1; Golgi adaptor HA1/AP1 adaptin subunit gamma-1; MGC18255. |
DATASHEET
Host:Rabbit
Target Protein:Gamma-Adaptin
IR:Immunogen Range:721-822/822
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:164
Swiss Prot:P22892
Source:KLH conjugated synthetic peptide derived from human Gamma-Adaptin:721-822/822
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta, beta prime and gamma adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
293T(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-Gamma-Adaptin (bs-1600R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kD
Observed band size: 91 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (bs-1600R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated(bs-1600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated(bs-1600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat brian; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Gamma-Adaptin, Unconjugated(bs-1600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Cell culture(MCF7); Paraformaldehyde-fixed; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (bs-1600R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes and DAPI staining.
Cell culture(SW480); Paraformaldehyde-fixed; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (bs-1600R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes and DAPI staining.
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