Anti-Phospho-IRF7 (Ser471 + Ser472) antibody (bs-3196R)

Anti-Phospho-IRF7 (Ser471 + Ser472) antibody

DATASHEET

Host:Rabbit

Target Protein:Phospho-IRF7 (Ser471 + Ser472)

IR:Immunogen Range:GV(p-S)(p-S)LD

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3665

Swiss Prot:Q92985

Source:KLH conjugated synthesised phosphopeptide derived from human IRF7 around the phosphorylation site of Ser471/472:GV(p-S)(p-S)LD 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
Flow-Cyt(1ug/test)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Uterus (Mouse) Lysate at 40 ug
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 49kD

Sample:Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 48kD

Sample:Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 49kD

Sample: Heart(Mouse) lysate at 30ug;
Primary: Anti-Phospho-IRF7 (Ser471+Ser472) (bs-3196R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 54kD
Observed band size : 51kD

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.