Anti-14-3-3 epsilon antibody
| 英文名称 | 14-3-3 epsilon |
| 中文名称 | 14-3-3E蛋白抗体 |
| 别 名 | 14-3-3 epsilon; 14 3 3 E; 14 3 3 epsilon; 14 3 3E; 14-3-3 E; 14-3-3 protein epsilon; 14-3-3E; 1433E_HUMAN; KCIP 1; KCIP-1; KCIP1; MDCR; MDS; mitochondrial import stimulation factor L subunit; protein kinase C inhibitor protein-1; Protein kinase C inhibitor protein1; Tyrosine 3 monooJNCgenase/tryptophan 5 monooJNCgenase activation protein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooJNCgenase activation protein epsilon polypeptide; Tyrosine 3/tryptophan 5 monooJNCgenase activation protein epsilon polypeptide; YWHAE. |
DATASHEET
Host:Rabbit
Target Protein:14-3-3 epsilon
IR:Immunogen Range:151-255/255
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:7531
Swiss Prot:P62258
Source:KLH conjugated synthetic peptide derived from human 14-3-3E:151-255/255
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Cow
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- 14-3-3 epsilon (bs-2340R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Sample:
A431(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti- 14-3-3 epsilon (bs-2340R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Sample:
Hela Cell (Human) Lysate at 40 ug
HT1080 Cell (Human) Lysate at 40 ug
Primary: Anti-14-3-3 epsilon (bs-2340R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- 14-3-3 epsilon (bs-2340R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (14-3-3 epsilon) Polyclonal Antibody, Unconjugated (bs-2340R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (14-3-3 epsilon) Polyclonal Antibody, Unconjugated (bs-2340R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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