Anti-phospho-HSP70 (Tyr41) antibody
| 英文名称 | phospho-HSP70 (Tyr41) |
| 中文名称 | 磷酸化热休克蛋白-70抗体 |
| 别 名 | HSP70 (phospho 41); HSP70 (phospho Y41); p-HSP70 (Tyr41); p-HSP70 (Y41); HSP70; HSP-70; HSP 70; Heat shock 70 kDa protein 1; heat shock 70kDa protein 1A; Heat shock 70kDa protein 1B; Heat shock induced protein; heat shock protein 70; HSP70 1; HSP70 2; HSP70.1; HSP72; HSPA1; HSPA1A; HSPA1B; XXbac BCX40G17.3 001; Heat shock 70 kDa protein 1A/1B; HSP71A_HUMAN; Hspa1a; Hsp70-1; HSP72; Hspa1; Hspa1b; Heat shock 70 kDa protein 1A/1B; shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2. |
DATASHEET
Host:Rabbit
Target Protein:phospho-HSP70 (Tyr41)
IR:Immunogen Range:PS(p-Y)VA
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3303
Swiss Prot:P0DMV8
Source:KLH conjugated synthesised phosphopeptide derived from human HSP70 around the phosphorylation site of Tyr41:PS(p-Y)VA
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Epithelium (Mouse) Lysate at 40 ug
Primary: Anti-phospho-HSP70 (Tyr41) (bs-10451R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD
Sample:
A549(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-HSP70 (Tyr41) (bs-10451R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD
Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-HSP70(Tyr41)Polyclonal Antibody, Unconjugated(bs-10451R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-HSP70 (Tyr41)) polyclonal Antibody, Unconjugated (bs-10451R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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