Anti-ADRA1A antibody
| 英文名称 | ADRA1A |
| 中文名称 | alpha 1肾上腺素能受体A抗体 |
| 别 名 | ADA1A_HUMAN; Adrenergic alpha 1A receptor; Adrenergic alpha 1C receptor; Adrenergic alpha 1D receptor; alpha 1 Adrenergic Receptor; Alpha 1A adrenergic receptor; Alpha-1A adrenergic receptor; Alpha-1A adrenoreceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c; ADRA1A; ADRA1C; Alpha 1A adrenoceptor; alpha-1A adrenergic receptor isoform 1; adrenergic, alpha-1A-, receptor variant 1; adrenergic, alpha-1A-, receptor variant 3; adrenergic, alpha-1A-, receptor variant 5; adrenergic, alpha-1A-, receptor variant 8; G protein coupled receptor; alpha-1A adrenoceptor; ADRA1L1; ALPHA1AAR. |
DATASHEET
Host:Rabbit
Target Protein:ADRA1A
IR:Immunogen Range:201-300/466
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:148
Swiss Prot:P35348
Source:KLH conjugated synthetic peptide derived from human Alpha-1A adrenergic receptor:201-300/466
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg /test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
U937 Cell (Human) Lysate at 30 ug
Raji Cell (Human) Lysate at 30 ug
Primary: Anti-ADRA1A (bs-0600R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 55 kD
Sample:
kidney (Mouse) Lysate at 40 ug
Primary: Anti-ADRA1A (bs-0600R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 62 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-ADRA1A (bs-0600R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 62 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRA1/ADRA1B/alpha 1 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-0600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRA1/ADRA1B/alpha 1 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-0600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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