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Anti-E-Selectin antibody (bs-1273R)

Anti-E-Selectin antibody (bs-1273R)

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Anti-E-Selectin antibody

英文名称E-Selectin
中文名称E选择素抗体
别    名CD 62E; CD26E; CD62 antigen-like family member E; CD62E; CD62E antigen; E selectin; E-selectin; ELAM 1; ELAM; ELAM-1; ELAM1; Endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; LECAM 2; LECAM2; Leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; LYAM2_HUMAN; SELE; Selectin E; SelectinE.  

DATASHEET

Host:Rabbit

Target Protein:E-Selectin

IR:Immunogen Range:501-612/612

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:20339

Swiss Prot:Q00690

Source:KLH conjugated synthetic peptide derived from mouse E-Selectin:501-612/612 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The endothelial leukocyte adhesion molecule-1 is expressed by cytokine-stimulated endothelial cells. It is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. This gene is present in single copy in the human genome and contains 14 exons spanning about 13 kb of DNA. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Siha Cell Lysate at 40 ug
Primary: Anti- E-Selectin (bs-1273R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD

Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti- E-Selectin (bs-1273R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD

Sample: HL-60 Cell (Human) Lysate at 40 ug
Primary: Anti- E-Selectin (bs-1273R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD

Sample:
DU145 Cell Lysate at 40 ug
LOVO Cell Lysate at 40 ug
Primary: Anti-E-Selectin (wt-p53)(bs-1273R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 63 kD

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