Anti-PALB2 antibody (bs-0588R)

Anti-PALB2 antibody

DATASHEET

Host:Rabbit

Target Protein:PALB2

IR:Immunogen Range:1101-1186/1186

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:79728

Swiss Prot:Q86YC2

Source:KLH conjugated synthetic peptide derived from human PALB2:1101-1186/1186 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a protein that may function in tumor suppression. This protein binds to and colocalizes with the breast cancer 2 early onset protein (BRCA2) in nuclear foci and likely permits the stable intranuclear localization and accumulation of BRCA2. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteosome-mediated degradation.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
MDA-MB-231(Human) Cell Lysate at 40 ug
Primary: Anti-PALB2 (bs-0588R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131 kD

Sample:
HepG2(Human) Cell Lysate at 40 ug
Primary: Anti-PALB2 (bs-0588R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131 kD

Independently Validated Antibody, image provided by Science Direct, badge number 029682:Formalin-fixed and paraffin embedded pig skeletal muscle labeled with Rabbit Anti-PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:100 for one hour at room temperature followed by conjugation to the secondary antibody Mach2 rabbit HRP-Polymer for 30 min at room temperature and DAB staining.

Independently Validated Antibody, image provided by Science Direct, badge number 029682:Formalin-fixed and paraffin embedded human breast cancer labeled with Rabbit Anti-PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:100 from one hour at room temperature followed by conjugation to the secondary antibody Mach2 rabbit HRP-Polymer for 30 minutes at room temperature. Positive Control, the staining is correct.

Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PALB2 Polyclonal Antibody, Unconjugated(bs-0588R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining