Anti-OCLN antibody (bs-1495R)

Anti-OCLN antibody

DATASHEET

Host:Rabbit

Target Protein:OCLN

IR:Immunogen Range:431-522/522

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:18260

Swiss Prot:Q61146

Source:KLH conjugated synthetic peptide derived from mouse OCLN:431-522/522 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(2μg/Test)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Guinea Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: A549 Cell Lysate at 30 ug
Primary: Anti- OCLN (bs-1495R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD

Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: MCF-7 (Human) Cell Lysate at 30 ug
Lane 3: A431 (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Lane 5: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-OCLN (bs-1495R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65/55/38/28 kD
Observed band size: 65/38/28 kD

Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-OCLN (bs-1495R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD

Sample: A549 Cell Lysate at 30 ug
Primary: Anti- OCLN (bs-1495R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 60 k