Anti-GST tag antibody (bs-2122R)

Anti-GST tag antibody

DATASHEET

Host:Rabbit

Target Protein:GST tag

IR:Immunogen Range:

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:N/A

Swiss Prot:N/A

Source:Recombinant full length GST tag protein: 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:GST is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:20000-60000)
ELISA(1:5000-10000)

Cross Reactive Species:GST fusion protein.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
GST-Tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 4 ug
Primary:
Anti-GST tag (bs-2122R) at 1/60000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD

Sample:
GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 4 ug
Primary: Anti-GST Tag (bs-2122R) at 1/1000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD

Sample:
GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2 ug
Primary: Anti-GST Tag (bs-2122R) at 1/1000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD

Sample:
Lane 1: GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
Lane 2: GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
Primary: Anti-GST tag (bs-2122R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

Sample:
Lane1: GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug
Lane2: GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug
Primary: Anti-GST tag (bs-2122R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD

Sample:
Recombinant GST Protein Lysate at 40 ug
Primary: Anti-GST tag (bs-2122R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 27 kD