Anti-StAR antibody (bs-3570R)

Anti-StAR antibody

DATASHEET

Host:Rabbit

Target Protein:StAR

IR:Immunogen Range:101-200/285

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6770

Swiss Prot:P49675

Source:KLH conjugated synthetic peptide derived from human StAR/StARD1:101-200/285 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(3μg /Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Adrenal gland(mouse) Lysate at 40 ug
Primary: Anti-StAR (bs-3570R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32kD
Observed band size: 32 kD

Sample:
Ovary (Mouse) Lysate at 30 ug
Brain (Mouse) Lysate at 30 ug
Primary: Anti-STAR/STARD1(bs-3570R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 32kD
Observed band size : 32kD

Sample:
Lane 1: Ovary (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Kidney (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: K562 (Human) Cell Lysate at 30 ug
Lane 6: THP-1 (Human) Cell Lysate at 30 ug
Lane 7: U937 (Human) Cell Lysate at 30 ug
Lane 8: HepG2 (Human) Cell Lysate at 30 ug
Primary:
Anti-StAR (bs-3570R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 27 kD

Sample:
K562(human) cell Lysate at 30 ug
Primary: Anti-StAR (bs-3570R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32kD
Observed band size: 32 kD

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (StAR) Polyclonal Antibody, Unconjugated (bs-3570R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.