Anti-phospho-HMGCR (Ser872) antibody
| 英文名称 | phospho-HMGCR (Ser872) |
| 中文名称 | 磷酸化三羟基三甲基辅酶A还原酶抗体 |
| 别 名 | phospho-HMGCR (S872); HMGCR (phospho-Ser872); HMGCR (phospho S872); 3 hydroJNC 3 methylglutaryl CoA reductase; 3 hydroJNC 3 methylglutaryl Coenzyme A reductase; 3 hydroJNCmethylglutaryl CoA reductase; 3-hydroJNC-3-methylglutaryl CoA reductase (NADPH); 3-hydroJNC-3-methylglutaryl-coenzyme A reductase; 3H3M; HMDH_HUMAN; HMG CoA reductase; HMG CoAR; HMG-CoA reductase; HydroJNCmethylglutaryl CoA reductase; LDLCQ3; MGC103269; Red. |
DATASHEET
Host:Rabbit
Target Protein:phospho-HMGCR (Ser872)
IR:Immunogen Range:NR(p-S)KI
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3156
Swiss Prot:P04035
Source:KLH conjugated Synthesised phosphopeptide derived from human HMGCR around the phosphorylation site of Ser872:NR(p-S)KI
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/Test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- phospho-HMGCR (Ser872) (bs-4063R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 97 kD
Observed band size: 97 kD
Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-HMGCR (Ser872) (bs-4063R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 97 kD
Observed band size: 97 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (phospho-HMGCR (Ser872)) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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