Anti-MST3/STK3 antibody
| 英文名称 | MST3/STK3 |
| 中文名称 | 丝氨酸/苏氨酸蛋白激酶3抗体 |
| 别 名 | Kinase responsive to stress 1; KRS1; Mammalian STE20 like protein kinase 2; Mammalian sterile 20-like 2; Mess1; MST; MST-2; MST2; Mst3; Serine/threonine kinase 3 (STE20 homolog, yeast); Serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine protein kinase 3; Serine/threonine protein kinase Krs1; STE20 like kinase MST2. |
DATASHEET
Host:Rabbit
Target Protein:MST3/STK3
IR:Immunogen Range:101-200/491
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:8428
Swiss Prot:Q9Y6E0
Source:KLH conjugated synthetic peptide derived from human STK3:101-200/491
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:STK3 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Muslce (Mouse) Lysate at 40 ug
Fetal brain (Mouse) Lysate at 40 ug
Primary: Anti- MST3/STK3 (bs-7599R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- MST3/STK3 (bs-7599Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 52 kD
Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: Anti- MST3/STK3 (bs-7599R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 53 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Bladder (Mouse) Lysate at 40 ug
Primary: Anti- MST3/STK3 (bs-7599R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MST3 STK3) Polyclonal Antibody, Unconjugated (bs-7599R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-STK3/MST3 Polyclonal Antibody, Unconjugated(bs-7599R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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