Anti-MUL1 antibody
| 英文名称 | MUL1 |
| 中文名称 | E3泛素连接酶MUL1抗体 |
| 别 名 | E3 ubiquitin-protein ligase MUL1; C1orf166; E3 ubiquitin ligase; E3 ubiquitin protein ligase MUL1; GIDE; Growth inhibition and death E3 ligase; MAPL; Mitochondrial anchored protein ligase; Mitochondrial ubiquitin ligase activator of NFKB 1; MUL1; MULAN; Putative NF kappa B activating protein 266; RING finger protein 218; RNF218; RP23-25C1.10-002; MUL1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:MUL1
IR:Immunogen Range:1-100/352
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:79594
Swiss Prot:Q969V5
Source:KLH conjugated synthetic peptide derived from human MUL1/RNF218:1-100/352
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:E3 ubiquitin-protein ligase that plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugatin.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/test)
IF(1:50-200)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti-MUL1 (bs-9291R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40kD
Observed band size: 40kD
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUL1) Polyclonal Antibody, Unconjugated (bs-9291R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUL1) Polyclonal Antibody, Unconjugated (bs-9291R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295g-cy3) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUL1) Polyclonal Antibody, Unconjugated (bs-9291R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295g-cy3) for 90 minutes, and DAPI for nuclei staining.
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