Anti-VAX1 antibody (bs-11496R)

Anti-VAX1 antibody

DATASHEET

Host:Rabbit

Target Protein:VAX1

IR:Immunogen Range:133-200/334

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:11023

Swiss Prot:Q5SQQ9

Source:KLH conjugated synthetic peptide derived from human VAX1:133-200/334 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The homeobox DNA-binding domain is a 60 amino acid motif that is conserved among many species and functions to bind DNA via a helix-turn-helix structure, thereby playing a role in transcriptional regulation and the control of gene expression. VAX1 (ventral anterior homeobox 1) is a 334 amino acid protein that localizes to the nucleus and contains one homeobox DNA-binding domain. Expressed as multiple alternatively spliced isoforms, VAX1 is required for major tract formation and axon guidance in the developing brain and may play a role in the differentiation of various structures, including the optic stalk, the neuroretina and the pigmented epithelium. The gene encoding VAX1 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(0.2μg/Test)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
MCF-7 Cell (Human) Lysate at 40 ug
U937 Cell (Human) Lysate at 40 ug
Primary: Anti-VAX1 (bs-11496R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 36 kD

Sample: BRL-3A Cell (Rat) Lysate at 40 ug
Primary: Anti- VAX1 (bs-11496R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 37 kD

Sample: RAW264.7 Cell (Mouse) Lysate at 40 ug
Primary: Anti- VAX1 (bs-11496R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 37 kD

Sample: NIH/3T3 Cell (Mouse) Lysate at 40 ug
Primary: Anti- VAX1 (bs-11496R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 37 kD