Anti-OTUD1 antibody (bs-17563R)

Anti-OTUD1 antibody

DATASHEET

Host:Rabbit

Target Protein:OTUD1

IR:Immunogen Range:141-240/481

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:220213

Swiss Prot:Q5VV17

Source:KLH conjugated synthetic peptide derived from human OTUD1:141-240/481 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA7 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.[supplied by OMIM, May 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Testis (Mouse)Lysate at 40 ug
Primary: Anti-OTUD1(bs-17563R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 60kD

Sample: HepG2 (Human) Cell Lysate at 40 ug
Primary: Anti-OTUD1(bs-17563R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 60kD

Sample: 293T (Human)Cell Lysate at 40 ug
Primary: Anti-OTUD1(bs-17563R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 60kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (bs-17563R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (bs-17563R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (bs-17563R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.