Anti-OPG antibody (bs-20624R)

Anti-OPG antibody

DATASHEET

Host:Rabbit

Target Protein:OPG

IR:Immunogen Range:201-300/401

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4982

Swiss Prot:O00300

Source:KLH conjugated synthetic peptide derived from human OPG:201-300/401 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Osteoprotegerin (OPG, or osteoclastogenesis inhibitory factor) is a secretory glycoprotein belonging to TNF receptor superfamily. Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. OPG acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis with increased osteoclast activity. OPG consists of 401 amino acids with a molecular weight of 44 kDa as a monomer and 90 kDa as a disulphide-linked dimer.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Raji (Human) Cell Lysate at 30 ug
Lane 2: U2os (Human) Cell Lysate at 30 ug
Lane 3: Heart (Mouse) Lysate at 40 ug
Lane 4: Heart (Rat) Lysate at 40 ug
Lane 5: Liver (Mouse) Lysate at 40 ug
Lane 6: Liver (Rat) Lysate at 40 ug
Lane 7: Kidney (Mouse) Lysate at 40 ug
Lane 8: Kidney (Rat) Lysate at 40 ug
Primary: Anti-OPG (bs-20624R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Sample:
Lane1:Heart(Mouse)Lysate at 40 ug
Lane2: Kidney(Mouse) Lysate at 40 ug
Lane3: Lung(Mouse) Lysate at 40 ug
Primary: Anti-OPG(bs-20624R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 44kD
Observed band size: 54kD

Sample: Lung (Mouse)Lysate at 40 ug
Primary: Anti-OPG(bs-20624R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 44kD
Observed band size: 54kD

Sample: Raji (Human) Cell Lysate at 40 ug
Primary: Anti-OPG(bs-20624R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 44kD
Observed band size: 54kD

Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OPG) Polyclonal Antibody, Unconjugated (bs-20624R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OPG) Polyclonal Antibody, Unconjugated (bs-20624R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.