Anti-AMPK alpha 1 antibody
| 英文名称 | AMPK alpha 1 |
| 中文名称 | 腺苷单磷酸活化蛋白激酶α1抗体 |
| 别 名 | AMPK alpha-1; 5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; AAPK1; acetyl CoA carboJNClase kinase; AI194361; AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha -1; AMPK 1; AMPK alpha 1 chain; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; cb116; EC 2.7.11.1; HMG CoA reductase kinase;hormone sensitive lipase kinase; im:7154392; kinase AMPK alpha1; MGC33776; MGC57364; PRKAA 1; PRKAA1; Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; wu:fa94c10; AAPK1_HUMAN |
DATASHEET
Host:Rabbit
Target Protein:AMPK alpha 1
IR:Immunogen Range:351-450/559
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5562
Swiss Prot:Q13131
Source:KLH conjugated synthetic peptide derived from human AMPK alpha 1:351-450/559
Purification:affinity purified by Protein G
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:200-1000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(3ug/Test)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
HepG2(Human) Cell Lysate at 40 ug
Primary: Anti-AMPK alpha 1 (bs-1115R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
Sample:
Hela(Human) Cell Lysate at 40 ug
Primary: Anti-AMPK alpha 1 (bs-1115R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
Tissue/cell: mouse colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AMPK alpha 1/PRKAA1 Polyclonal Antibody, Unconjugated(bs-1115R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AMPK alpha 1/PRKAA1 Polyclonal Antibody, Unconjugated(bs-1115R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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