Anti-ODC antibody (bs-1294R)

Anti-ODC antibody

DATASHEET

Host:Rabbit

Target Protein:ODC

IR:Immunogen Range:321-461/461

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4953

Swiss Prot:Q9BQT8

Source:KLH conjugated synthetic peptide derived from human ODC:321-461/461 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Ornithine DecarboJNClase (ODC) is the initial and rate limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Kidney(Mouse) lysate at 30ug;
Primary: Anti-ODC(bs-1294R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:51 kD
Observed band size:51 kD

Sample:Liver(Mouse) lysate at 30ug;
Primary: Anti-ODC(bs-1294R) at 1:300 dilution;
Secondary: AP conjugated Goat Anti-Rabbit IgG(bs-0295G-AP) at 1: 5000 dilution;
Predicted band size : 51kD
Observed band size : 51kD

Sample: Kidney(Mouse) lysate at 30ug;
Primary: Anti-ODC(bs-1294R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:51 kD
Observed band size:51 kD

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (bs-1294R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (bs-1294R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.