Anti-Mtp53(N235K N239Y) antibody (bs-0913R)

Anti-Mtp53(N235K N239Y) antibody

DATASHEET

Host:Rabbit

Target Protein:Mtp53(N235K N239Y)

IR:Immunogen Range:201-300/393

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7157

Swiss Prot:P04637

Source:KLH conjugated synthetic peptide derived from human Mtp53 arund the N235K N239Y:201-300/393 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The widely studied P53 tumor suppressor gene has been found to contain mutations in over 50% of human cancers. The level of P53 protein is low in normal cells, but is increased in response to DNA damage or various other cellular distress signals. Overexpression of the P53 transcription factor can induce ether cell cycle arrest or apoptosis through transcriptional regulation of several genes including the cell cycle ingibitor P21, DNA repair gene GADD45, and the apoptotic inducer Bax. P53 has also been shown to induce apoptosis by meas of s direct signaling pathway. P53 directly binds to and acts on several cellular protein involved in various pathway, including c-Abl, basal transcription factor TFIIH and WT1. P53 can be functionally inactivated by mutation, binding to DNA tumor virus encoded proteins, such as SV40 large T antigen, Adenovirus E1B and papilloma virus E6 proteins or as a consequence of its interaction with the oncogene-encoded protein MDM2. This antibody is recognize the Human P53 Core Domain Mutant N235KN239Y.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mtp53(N235K N239Y) ) Polyclonal Antibody, Unconjugated (bs-0913R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: human ovary carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Mtp53(N235K N239Y) Polyclonal Antibody, Unconjugated(bs-0913R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining