Anti-CD57 antibody
| 英文名称 | CD57 |
| 中文名称 | 髓鞘相关糖蛋白CD57抗体 |
| 别 名 | 3-glucuronyltransferase 1; 3-glucuronyltransferase; B3GA1_HUMAN; B3GAT1; Beta 1 3 glucuronyltransferase 1; Beta-1; CD 57; CD57; CD57 antigen; GalactosylgalactosylJNClosylprotein 3 beta glucuronosyltransferase 1; GalactosylgalactosylJNClosylprotein 3-beta-glucuronosyltransferase 1; GlcAT P; GlcAT-P; GLCATP; GlcUAT P; GlcUAT-P; GlcUATP; Glucuronosyltransferase P; HNK 1; HNK1; LEU 7; LEU7; LEU7 antigen; NK 1; NK1; UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase; UDP-GlcUA:glycoprotein beta-1. |
DATASHEET
Host:Rabbit
Target Protein:CD57
IR:Immunogen Range:21-120/334
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:27087
Swiss Prot:Q9P2W7
Source:KLH conjugated synthetic peptide derived from human CD57:21-120/334
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:CD57 recognizes a (Mr 100-110kDa) oligosaccharide antigenic determinant on myeloid cells and on a variety of polypeptides, lipids and chondroitan sulfate proteoglycans. This surface antigen is associated with myelin-associated glycoprotein (MAG). The CD57 antigen is present on 15-20% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of T-lymphocytes. This carbohydrate is also present on N-CAM in the nervous system.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-CD57 (bs-1635R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 63 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-CD57 (bs-1635R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 63 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Brain (Mouse) Lysate at 40 ug
U-87MG (Human) Cell Lysate at 30 ug
Primary: CD57(bs-1635R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 63 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD57) Polyclonal Antibody, Unconjugated (bs-1635R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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